These metabolome samples were from peanut leaves.

The yield and quality of peanut (Arachis hypogaea L.), which is a dominant oil crop widely planted, are often limited by drought stress (DS) and nitrogen (N) deficiency. To investigate the molecular mechanism of peanut counteracting N deficiency and drought, transcriptomic and metabolomic analyses of peanut leaves were executed in this study and the results demonstrated that N defi-ciency and DS significantly inhibit peanut growth and pod yield. Herein, 3867 known differentially accumulated metabolites (DAMs), 324 differentially expressed transcription factors (TFs), and 7137 differentially expressed genes (DEGs) were recognized in peanut leaves under different nitrogen and water conditions. Metabolomic analysis showed that metabolic accumulation in these pathways was significantly dependent on the stress conditions. Additionally, the role of metabolites and genes in pathways such as biosynthesis of AAs and phenylpropanoid biosynthesis under different stress conditions were discussed. The results demonstrated that different genes, metabolic pathways, metabolites were related to N deficiency and drought. The study provides metabolic pathways and functional genes that can be used for improvement of peanut resistance to abiotic stress.