HNF4a regulates hepatic peroxisomal biogenesis and fatty acid elongation during the thermogenic response

We observed gene expression changes in livers of cold exposed mice along with changes in global genome occupancy for the transcription factor HNF4a, RNA polymerase II and the histone marker H3K27Ac, suggesting a hepatic transcriptional response to cold xposure. Overall design: Performed RNA-seq for gene expression changes on livers of cold exposed mice during a 24 hour time course (hours 0,6,12,24). Perforemed ChIP-seq for HNF4a, Rpb1-CTD, H3K27Ac on livers from room temperture and cold exposed (6 hours) mice.