Transcriptome profiling of kidney medulla of Actr3 KO mice

Renal tubule-specific Actr3 knockout mice (Actr3-fl/fl*Cdh16-Cre) were generated. Transcriptome profiling (RNA-Seq) and differential gene expression analysis of isolated renal medulla from KO and WT mice at p14 was performed. Actr3 KO mice showed transcriptional changes associated with Actr3 loss and kidney disease. mRNA sequencing analysis of kidney medulla of WT control and Actr3-fl/fl*Cdh16-Cre mice成功构建肾小管特异性Actr3基因敲除小鼠（Actr3-fl/fl*Cdh16-Cre）。对出生后第14天（postnatal day 14, p14）的敲除（Knock Out, KO）与野生型（Wild Type, WT）小鼠分离得到的肾髓质，开展了转录组测序（RNA-Seq）及差异基因表达分析。结果表明，Actr3敲除小鼠呈现出与Actr3基因缺失及肾脏疾病相关的转录组学改变。本研究针对野生型对照及Actr3-fl/fl*Cdh16-Cre小鼠的肾髓质完成了mRNA测序分析。