Eed, a member of the Polycomb group, is required for nephron differentiation and the maintenance of nephron progenitor cells

We used R26R-tdTomato reporter mice activated by the Six2-TGC transgene to isolate cell populations co-expressing GFP and tdTomato by FACS from kidneys of control P0 mice. We observed two distinct populations of cells from control kidneys based on lower or higher tdTomato expression along with high GFP-expression from the Six2-TGC transgene. RNASeq experiment was conducted to analyze the transcriptome of these populations Examination of 2 different populations of cells derived from Six2-expressing kidney by RNA-seq.我们采用经Six2-TGC转基因（Six2-TGC transgene）激活的R26R-tdTomato报告基因小鼠（R26R-tdTomato reporter mice），通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting，FACS）从对照组出生后0日龄（P0）小鼠的肾脏组织中分离出共表达绿色荧光蛋白（Green Fluorescent Protein，GFP）与tdTomato荧光蛋白（tdTomato）的细胞群。基于tdTomato荧光蛋白的表达水平高低，结合Six2-TGC转基因介导的高GFP表达特征，我们从对照小鼠肾脏中观察到两类截然不同的细胞群。本研究开展了RNA测序（RNASeq）实验以分析上述细胞群的转录组，并通过RNA测序对源自Six2阳性肾脏细胞的两种不同细胞群进行了转录组检测。