Proteomic analysis of secretomes from <i>Bacillus</i> sp. AR03: characterization of enzymatic cocktails active on complex carbohydrates for xylooligosaccharides production

<i>Bacillus</i> sp. AR03 have been described as an important producer of carbohydrate-active enzymes (CAZymes) when growing in a peptone-based medium supplemented with simple sugars and/or carboxymethyl cellulose (CMC) as carbon sources. This work aimed to identify the extracellular enzymatic cocktails through shotgun proteomics. The proteomic analysis showed that enzymes involved in cellulose and xylan degradation were among the most abundant proteins. These enzymes included an endo-glucanase GH5_2 and a glucuronoxylanase GH30_8, which were found in all conditions. In addition, several proteins were differentially expressed in the three evaluated culture media, indicating microbial metabolic changes due to the different supplied carbon sources, particularly, in the presence of CMC. Finally, the capability of the crude enzymatic cocktails from culture media to degrade birchwood xylan was assessed, which produced mostly xylooligosaccharides containing among 3–5 xylose units. Consequently, this work shows the potential of the extracellular enzymes from <i>Bacillus</i> sp. AR03 for producing emergent prebiotics.芽孢杆菌属（Bacillus）菌株AR03被报道为一类重要的碳水化合物活性酶（carbohydrate-active enzymes, CAZymes）生产菌，当其在以单糖和/或羧甲基纤维素（carboxymethyl cellulose, CMC）为碳源的蛋白胨培养基中培养时。本研究通过鸟枪蛋白质组学（shotgun proteomics）技术，旨在鉴定该菌的胞外酶系。蛋白质组分析结果显示，参与纤维素与木聚糖降解的酶类属于丰度最高的蛋白组分，其中包括在所有培养条件下均被检测到的内切葡聚糖酶GH5_2与葡萄糖醛酸木聚糖酶GH30_8。此外，在三种受试培养基中存在多个差异表达的蛋白，表明不同碳源（尤其是添加CMC的培养条件）会引发微生物的代谢模式改变。最后，本研究评估了各培养基来源的粗酶系降解桦木木聚糖的能力，结果显示其主要生成包含3~5个木糖单元的低聚木糖（xylooligosaccharides）。综上，本研究证实了芽孢杆菌属AR03的胞外酶系在制备新兴益生元（prebiotics）方面的应用潜力。

搜集汇总

背景与挑战

背景概述

该数据集通过蛋白质组学分析，研究了Bacillus sp. AR03在不同碳源培养基中产生的胞外酶混合物，重点识别了纤维素和木聚糖降解酶（如GH5_2和GH30_8），并发现这些酶能有效生产木寡糖，具有作为益生元的应用潜力。数据集突出了微生物代谢对碳源的响应及其在生物技术中的价值。