RNA seq analysis of murine whole colon using Takara SMART-Seq Stranded kit. Libraries were sequenced on an Illumina NovaSeq 6000 to an approximate sequencing depth of 60 million total reads

The intestinal microbiota fundamentally influences the development of a normal intestinal physiology and education and functioning of the mucosal immune system. The goal of this study is to analyze how the transcriptional profile of the murine colon can be influence by colonization of gnotobiotic mice with specific bacterial strains . Overall design: Whole colon RNA profiles of GF (Germ free), OMM12 (Oligo mouse microbiota 12), OMM12 + MC2 (Oligo mouse microbiota 12 + minimal consortium 2) and SPF (specific pathogen free) mice