RNA-seq of seminal plasma exposure of bEND cells

The present study evaluated the impact of seminal plasma from bulls exposed to overnutrition on the transcriptome of endometrial epithelial (BEND) cells in vitro. Yearling half-siblings beef bulls (n = 20) were stratified by age, body weight, and body condition score before being randomly assigned to one of two diets: 1) a diet formulated to elicit a target ADG of 1.81 kg/d (High Gain, n = 10) or 2) a diet formulated to elicit a target ADG of 1.23 kg/d (Moderate Gain, n = 10). Seminal plasma was collected from each treatment group at three timepoints and pooled then passed through a 22 um sterile filter. Cells were randomly assigned to one of three treatment groups: no treatment (Control), 1% seminal plasma from moderate gain bulls (Moderate Gain), or 1% seminal plasma from high gain bulls (High Gain). Following the treatment period, total RNA was extracted from cells and sequenced.