Relative abundance of primary (A & D), secondary (B & E) and 6α-hydroxylated (C & F) bile acids in serum (A–C) and urine (D–F) from non-cholestatic donors and/or patients before and after biliary stenting.

Bile acids were quantified as indicated in the “Experimental Procedures” section. The relative abundance (expressed as a percentage) of primary, secondary and 6α-hydroxylated bile acid species was determined by adding up all unconjugated and/or conjugated species of CDCA+CA, LCA+DCA or HDCA+HCA, respectively, and by dividing the result by the total bile acids. Graphics represent the mean ± SEM (nM). P-values were determined by the rank sums Wilcoxon/Mann-Whitney test. *: p<0.05, ***: p<0.001. n.s.: not significant.