Gene expression data of organoids derived from traditional serrated adenomas

Traditional serrated adenomas (TSAs) are rare colorectal polyps characterized by unique histology features. Fusions in R-spondin genes have been found in TSAs, but it is not clear whether these are sufficient for TSA development. We established 2 patient-derived TSA organoid lines, and engineered chromosome rearrangements that involve R-spondin genes into human colonic organoids using CRISPR-Cas9. Consequently, we highlighted the similarity of the gene expression patterns between TSA organoids and engineered organoids. We analyzed gene expression of 2 patinet-derived traditional serrated adnoma, 3 genetically engineered and 1 normal colonic organoids. Total RNA were extracted from organoids and hybridized to GeneChip PrimeView Human Gene Expression Array, and scanned with GeneChip Scanner 3000 7G.