Additional file 2 of Visceral adiposity in postmenopausal women is associated with a pro-inflammatory gut microbiome and immunogenic metabolic endotoxemia

Supplementary Material 2: Supplemental Figure S1. Group 2 (Red box/blue circles): Highest Year 17 VAT +VAT gain since Year 5 subjects (n=25). Group 1 (Black box/red circles): Lowest Year 17 VAT +VAT loss since Year 5 subjects (n=25). 4 underweight (BMI<18.5; grey #) in lower left corner. Supplemental Figure S2. 3 participants had detectable levels of either amoxicillin (antiobiotic targeting Gram-positive bacteria) or clindamycin (antibiotic targeting both Gram-positive and Gram-negative bacteria) and were excluded from further analysis. Supplemental Figure S3. Read statistics. Metagenomic read depth varied from 8.4M to 39M per sample. Supplemental Figure S4. Anti- Lipoteichoic acid (LTA) IgA and anti-flagellin IgA levels increased significantly in the high VAT group. A. No significant difference in anti-LTA IgG levels between the VAT groups. B. High VAT group showed significantly higher anti-LTA IgA. C. No significant difference in the levels of anti-flagellin IgG between the VAT groups. D. Anti-flagellin IgA was significantly higher in the high VAT group. Supplemental Figure S5. Select adiposity-associated motile bacteria correlated with anti-flagellin IgA while no LTA-containing bacteria correlated with anti-LTA antibodies. A. Correlation matrix of LTA-containing bacteria that are significantly associated with body anthropometric measures. Bacteria are presented in ascending order of p value for association with BMI (p<0.05). No bacteria showed any correlation with anti-LTA antibodies. B. Correlation matrix of motile bacteria that are significantly associated with body anthropometric measures (p<0.05). Bacteria in bold are those that significantly correlated with both BMI and anti-flagellin IgA production.