Mid-gestation marker genes with increased mRNA expression levels in term placenta of pregnancy complications compared to placentas of uncomplicated gestationsa.

aMid-gestation marker genes with statistically non-significant results are given in Table S8.bCases included maternal pregnancy complications (preeclampsia, PE, n = 12; gestational diabetes mellitus, GDM, n = 12), fetal pregnancy complications (small-for-gestational-age, SGA, n = 12; large-for-gestational-age, LGA, n = 12), as well as control samples comprised of uncomplicated pregnancies resulting in the birth of an appropriate-for-gestational-age newborn (AGA, n = 12).cP-values from RT-qPCR data were estimated with Student t-test (no adjustment). For seven genes with significant t-test P-values, analysis of covariance (ANCOVA) adjusted by estimated confounder effects was used. All tests were adjusted by gestation age, placenta weight, infant gender and type of delivery. In case of GDM, tests were additionally adjusted by infant weight and maternal age, and with infant weight in case of PE. Bold letters highlight the genes with statistically significant values throughout analyses. T-test FDR correction considered 16 genes. ANCOVA FDR considered 7 genes.dFold change was calculated as the difference of mean relative expression values of each pregnancy complication vs control group of uncomplicated pregnancies.