Genetic Diversity Influences the Response of the Brain to Developmental Lead Exposure.

In this study we analyzed the effects of lead-exposure up hippocampal gene expression in males and females exposed to 0ppm, 250ppm and 750ppm lead during two different developmental periods, perinatal (in utero through to weaning at PND21) and postnatal (PND0-PND45), across three strains (Fischer, Long Evans and Sprague Dawley). All tissue was taken at PND 55. We used affymetrix Rat Gene 1.0ST arrays to obtain global gene expression data from each animal, with a group size of 4 for all conditions (Total number of Arrays = 119)本研究旨在分析铅暴露对大鼠海马体基因表达的影响。实验选取3种品系的大鼠——菲舍尔（Fischer）、朗埃文斯（Long Evans）及斯普拉格-道利（Sprague Dawley），分别在两种不同发育时期暴露于0ppm、250ppm与750ppm的铅环境中：其一为围产期（宫内至出生后第21天断奶，即出生后天数（Postnatal Day，PND）21），其二为产后阶段（出生后第0天至第45天，PND0-PND45）。所有组织样本均采集于出生后第55天（PND55）。本研究采用阿菲梅特里克斯（Affymetrix）大鼠基因1.0ST芯片，获取每只实验动物的全基因组基因表达数据；所有实验组的样本量均为4，总芯片使用量共计119张。