Prorhinotermes simplex caste transcriptome: de novo assembly, annotation and caste-specific expression data

Data supporting a study of caste-specific gene expression in the subterranean termite Prorhinotermes simplex, comparing established (old) kings, neotenic (young) kings and workers. The record contains: Psimplex_transcriptome_assembly.fasta.gz — de novo reference transcriptome, 256,870 transcripts (189–32,988 nt), assembled with Trinity and clustered with EvidentialGene. Sequence identifiers have the form evgLocus_N. Psimplex_transcriptome_annotation.tsv.gz — one row per transcript (256,870 rows) with transcript length, predicted ORF, best BLASTP hit against UniProt Swiss-Prot, Pfam domains and the identifier used in the count matrix. Psimplex_count_matrix_raw.tsv.gz — un-normalised read counts for 20,944 features across 27 libraries (10 established kings, 7 neotenic kings, 10 workers), obtained from QuantSeq 3′ mRNA sequencing after UMI deduplication. DESeq2_results.zip — DESeq2 results for the three pairwise caste contrasts (worker vs established king, neotenic king vs worker, neotenic king vs established king), 20,944 transcripts each. GO_enrichment.zip — GO term enrichment for the same three contrasts (405, 424 and 412 terms). ena_accessions.csv — mapping of library codes to ENA sample, experiment and run accessions. The underlying sequencing reads are deposited in the European Nucleotide Archive under study accession PRJEB129346.