T-cell receptor Vβ (TCRVB) deep sequencing on human T cells isolated from humanized mice engrafted with human PBMC and treated or not with PTCy post-transplantation

Nucleotide sequence data of  TCRVB sequencing performed on T cells isolated from the pre-transplantation hPBMC (donor T cells) or from mice organs at day 21 post-transplantation (injected or not with PTCy at day 3) to determine the impact of PTCy on the T cell V beta (TCRVB) receptor repertoire diversity.

NSG mice were engrafted with human PBMC to develop xeno-GVHD and treated or not with 100 mg/kg PTCy. Spleens and lungs from 10 NSG mice per group were pooled and stained to sorted  human CD4+ and CD8+ . DNA of human CD4+ and CD8+ T cells sorted from each organ and  from the PBMC donor was extracted. One hundred fifty µg from each sample were used for T-cell receptor Vβ (TCRVB) deep sequencing performed by Adaptive Biotechnologies.