Effect of a SNRPA1-interacting RNA structural element on alternative splicing. Effect of a SNRPA1-interacting RNA structural element on alternative splicing

Assessing the influence of RNA structural and sequence variations on SNRPA1-mediated regulation of alternative splicing Overall design: A SNRPA1-interacting RNA structural element (WT sequence hg38: chr8:143925142-143925188), along with sequence and structure mutants of this element, was transfected into human breast cancer cells and then RNA-seq was used to assess the resulting changes in alternative splicing. Mock-transfected cells were used as a control. The sequence of the WT and variant structural RNA are available in the file "structural_RNA_sequence_with_variants.txt" at the foot of this record.