miRNA expression profiling of mouse colon in chronic colitis and colitis-associated cancer models

This study aims to investigate alterations in colonic microRNA expression in mouse models of chronic colitis and colitis-associated colorectal cancer. Using the Affymetrix GeneChip miRNA 4.0 Array, miRNA profiles were analyzed from control mice, DSS-induced colitis mice, and AOM/DSS-induced colorectal tumor mice. A total of 20 mouse colon tissue samples were analyzed, including 6 control samples, 7 DSS-treated samples, and 7 AOM/DSS-treated samples. Total RNA was extracted and hybridized to the GeneChip miRNA 4.0 Array (Affymetrix). CEL and CHP files were generated for each sample and used for downstream analysis.