Cellular senescence in malignant cells promotes tumorigenesis in mouse and patient glioblastoma [RNA-seq]

We performed a scRNAseq analysis conducted at the endpoint of our GBM mouse model. We compared differentially expressed up-regulated genes in p16Ink4a positive vs p16Ink4a negative malignant cells. The goal was to compare this dataset with two other transcriptomic datasets, to explore the upstream regulator(s) of senescence in malignant cells. We found that Nuclear Factor Erythroid 2 Like 2 (Nfe2l2) signaling pathway was enriched in the 3 data sets. Overall design: ddSeq of a p16-3MR mouse GBM at end timepoints