Chromatin context and ncRNA highlight physiological targets of MECP2 in brain.

Analysis of chromatin and sequence context and RNA highlighting MeCP2 function in the genome ChIP-seq data uses 2 libraries for each sample comprising a bound and input reference, RNA-seq data uses MeCP2 null sample compared with 2 wild type replicate samples, Methylminer-seq data uses 1 high methylation sample and 1 low methylation sample, RNA-immunoprecipitated data uses 3 replicate samples.本数据集通过分析染色质、序列上下文及RNA，以阐明基因组中MeCP2的功能，所采用的实验数据详情如下：1. 染色质免疫共沉淀测序（ChIP-seq）数据：每个样本设置2个文库，分别为结合文库与输入对照文库；2. RNA测序（RNA-seq）数据：以MeCP2缺失样本作为实验组，与2个野生型生物学重复样本进行对照分析；3. Methylminer-seq数据：采用1个高甲基化样本与1个低甲基化样本开展实验；4. RNA免疫沉淀（RNA-immunoprecipitated）数据：使用3个生物学重复样本进行检测。