Genotype specific phenotypic behaviour of auxotrophic and prototrophic gene deletion collections

To systematically characterize the phenotypic behavior of two 'prototrophic' version of the Yeast Knockout (YKO) gene deletion collection, we compared genome-wide finess assays across a diverse set of drug and environmental conditions. This analysis uncovered experimental liabilities in the the prototrophic collections distinct from the YKO auxotrophies that shows that auxotrophic repair (regardless of methodology) does not restore wildtype behaviour.为系统性表征两株原养型（prototrophic）酵母敲除（Yeast Knockout, YKO）基因缺失文库的表型行为，我们针对多样化的药物与环境条件组合开展了全基因组适合度对比测定。本分析发现，原养型文库存在与YKO营养缺陷型不同的实验局限性，结果表明无论采用何种修复手段，营养缺陷型修复均无法恢复野生型的表型行为。