Cloning and variation of adult renal tubular progenitors

Kidney damage involves the progressive and inexorable destruction of tubular and glomerular system. However, it is known that the patients survive AKI often recover renal structure and function. Correspondingly, previous studies demonstrated tubular regeneration in mice after massive kidney injury and linked mouse Sox9+ renal progenitor cells to this process. Here we show that renal progenitor cells can be cloned from renal needle biopsy sample of CKD patients. Progenitor cells can readily assembly into “kidney organoids” expressing proximal/distal tubular cell markers in 3D culture. Tissue sample from CKD patients, isolated human Sox9+ renal tubular progenitors, and in vitro differentiated kidney organoids were prepared for RNA-sequencing. Duplicate experiments were taken from 2 biological samples.