Genome-wide maps of H3K27ac in HDAC3 knockout bone marrow pDCs compared with control pDCs

Hdac3 is highly expressed in pDCs, and its deficiency led to substantially impaired development of pDCs, but not cDCs in bone marrow (BM) and spleen. Meanwhile,further investigation demonstrated that HDAC3 was required for the differentiation of pDC from progenitors at different differentiation stages in vitro. Cut & Tag analysis of H3K27ac level in HDAC3 deficient bone marrow pDCs compared with control pDCs, showed that H3K27ac level significantly increased on cDC related genes with PU.1 binding sites in HDAC3 knockout pDCs. Overall design: Cut& Tag assay analyzed the H3K27ac level in HDAC3 knockout bone marrow pDCs compared with control pDCs.