The Set1 N-terminal domain and Swd2 interact with the RNA polymerase II CTD to recruit COMPASS

Methylation of histone H3 lysine 4 (H3K4) by the Set1/COMPASS complex is coupled with active transcription, and this modification is important for regulating gene expression. Set1/COMPASS associates with the RNA polymerase II (RNApII) C-terminal domain (CTD) to establish proper levels and distribution of H3K4 methylations.To ask how the Set1-RNApII CTD binding affects the occupancy and overall level of H3K4 methylations, ChIP-Seq was pefromed in cells transformed with full length or N-terminal truncated Set1. Our results indicate that Set1-RNApII CTD interaction is necessary for maintaining H3K4 methylations throughout the genes. 27 samples of H3K4me3, H3K4me2, FLAG ChIP-Seq for S.cerevisiae transformed with Full length or mutant Set1. Input samples from gene deletions were included.