Differential effects of estrogen receptor beta isoforms on glioblastoma progression

We examined the transcriptional changes modulated by knocking out ERß and reintroduction of ERß1 and ERß5 isoforms by perfroming global transcriptome analysis. ERß was knocked out in U87 cells using CRISPR/Cas9 system and reintroduced the ERß1 and ERß5 isoforms in knockout background. RNA was isolated from control U87, U87-ERß-KO, U87-ERß1 and U87-ERß5 cells and utilized for RNA-seq analysis. Our results demonstrated that ERß-KO, ERß1 and ERß5 modulated unique pathways including NF-?B singaling, Jak/STAT pathway and mTOR signaling. Overall design: Total RNA was isolated from U87 control, U87-ERß-KO, U87-ERß1 and U87-ERß5 cells. Illumina TruSeq RNA Sample Preparation was performed following manufacturer's protocol. Samples were run on an Illumina HiSeq 3000 in duplicates. The combined raw reads were aligned to UCSC hg19 and genes were annotated by Tophat. Genes were annotated and quantified by HTSeq-DESeq pipeline.