Camellia oleifera meal peptides alleviates atherosclerosis by regulating lipid metabolism through AMPK/ACC/CPT-1 pathway

Background: The potential of Camellia oleifera meal peptides(PP) in the treatment of atherosclerosis and its potential mechanism need to be further clarified. Objective: the aim of this study was to clarify the effect of PP in the treatment of atherosclerosis and its potential mechanism. Methods: After cell modeling, it was used to detect the antioxidant, anti-inflammatory and lipid-lowering effects of PP in vitro. As mouse model was used to evaluate the anti atherosclerotic effect of PP in vivo. The changes of protein and metabolites after the treatment of atherosclerosis by PP were studied by liver proteomics, metabonomics and transcriptomics, and the possible mechanism of its regulation of lipid metabolism was clarified. Results:Using cell inflammation model, oxidative damage model and high-fat model, CCK8 test and qPCR detection showed that PP had antioxidant, anti-inflammatory and lipid-lowering effects. The animal results showed that the lipid deposition in the root of aortic valve and liver tissue was reduced, and the PP had the effect of alleviating atherosclerosis. Metabonomics and proteomics analysis showed that the activities of various enzymes related to lipid metabolism were changed after PP treatment, and the lipid metabolism pathway was changed. Transcriptomics analysis showed that AMPK pathway was changed. The experimental results showed that AMPK regulated lipid oxidation pathway to alleviate atherosclerosis in mice through acetyl CoA carboxylase (ACC) - CPT-1 pathway. Overall design: AML12 cells in logarithmic growth phase and in good condition were seeded into 6-well and 96-well plates, with a seeding density of 1×106 cells/ml for the 6-well plate. AML12 cells were treated with PP and modeling reagent (PA/OA,400 nM/800 nM)24 hours. The experiment was divided into 3 groups: a blank group (control group, cultured in regular complete medium), a model group, and groups treated with PP (final concentrations of 250 µg/ml in PP-containing medium).