RNA-Seq analysis EIF5A2 knockdown effect on ovarian cancer cells

Eukaryotic initiation factor 5A2 (EIF5A2) acts as a candidate oncogene and is located on human chromosome 3q26, a region frequently amplified in several tumors. Accumulating evidence shows that EIF5A2 plays important roles in enhancing anchorage-independent growth, xenograft tumour growth, increasing cancer cell metastasis and promoting treatment resistance through multiple pathways. Here, we showed that EIF5A2 knockdown reduced the expression of KLF4, which could partially rescue stem-like properties abolished by EIF5A2 knockdown or strengthened by EIF5A2 overexpression, through the transcription factor E2F1, which directly bind to KLF4 promoter. Si-EIF5A2 or NC were transfected into SKOV3 cells (n=3 for each group) for 48 hours. Total RNA was isolated using Trizol reagent (Invitrogen, USA). Agilent 2100 Bioanalyzer (Agilent Technologies, USA) was used to evaluate the RNA quality.