Transcriptome profiles of seminiferous tubes on different stage of spermatogenesis

In order to get the different gene expression profiles of these seminiferous tubules on different stages of spermatogenesis, four groups of samples were taken in accordance with the differentiation stage of germ cells, namely: empty tubes before SSCs transplantation (named as “EmptBtrans”), empty tubes after SSCs transplantation (named as “EmptAtrans”), tubes which SSCs had successfully homed and differentiated into spermatocytes and round spermatids (named as “SM_and_RS”) and fluorescent tubes which were taken from recipient mice that already had offsprings to make sure these tubes had complete spermatogenesis (named as “sperm”). Pairwise comparison was made between these four groups, the number of significantly differentially expressed genes increased with the differentiation of germ cells. Overall design: Compare the gene expression profiles of different seminiferous tubules containing different kinds of germ cells