U0126 reverses the cardioprotective effect of HCQ in simulated I/R injury in neonatal rat cardiomyocytes.

Western blot analysis of cell lysates from neonatal rat cardiomyocytes pre-treated overnight with 2000 ng/mL HCQ and the following day incubated with 10 μM U0126 for 2 hours followed by simulated I/R injury (4h hypoxia + 2h reoxygenation). Immunoblots were probed for cleaved caspase-3, ERK1/2 phosphorylation (A) and ERK5 phosphorylation (B). Graphs show mean ±SEM of quantitative analysis from four independent experiments. Statistical analysis determined by 1 way ANOVA using post-hoc Tukey to compare all columns (*p<0.05, *** p<0.0005)(a).