Dataset: Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome. Boehm et al

This dataset contains the imaging source data for the manuscript titled "Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome" by Boehm et al. published in Molecular Cell in 2025.

The unprocessed and uncompressed imaging data (western blot and RT-PCR gels) for each main and supplemental figure can be found in the respective figure folder. For RT-PCR images, TIF (.tif) and raw Bio-Rad Image Lab Image Document (.scn) files are provided. For Western blots, multi-page TIFs (pages: 1. overlay, 2. raw chemiluminescence, 3. marker image, 4. final) are provided. Markers for western blots: PageRuler Prestained Protein Ladder (ThermoFisher Cat# 26617) and for RT-PCR: HyperLadder 1kb (Meridian Cat# BIO-33026).

The PDF file "Boehm et al Overview" provides an overview of all main and supplemental figure images used in this study.