five

The fate switch between ON and OFF SACs is determined by Fezf1

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https://www.ncbi.nlm.nih.gov/sra/SRP201013
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The goal of this experiment was to define genes differentially expressed between E17.5 Fezf1Hete and Fezf1KO SAC cells, which were isolated by FACS from mice retinae. Overall design: E17.5 Fezf1Hete and Fezf1KO SACs were isolated by FACS in pools of 100 cells. 13 Fezf1Hete and Fezf1KO replicates were collected from Fezf1GFP/+ and Fezf1GFP/GFP retinas, repectively. cDNA libraries were prepared with Smart-seq2 and Nextera XT (Picelli et al., 2014). Libraries were sequenced on the Illumina NextSeq with paired-end 75bp reads.
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2020-03-05
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