Single-cell RNA sequencing in Kawasaki disease

We performed single-cell RNA sequencing (using Cell Ranger Single Cell Pipeline v.4.0.0 to process data de-multiplexing, barcode processing, and single cell 3' gene counting generated by the 10X Chromium platform) in KD patients (within 24 hours before IVIG treatment) and age-matched fever controls. Overall design: Peripheral blood samples were collected from five KD patients (include three KD patients with following development of coronary artery lesions) within 24 hours before IVIG treatment, two febrile controls and two healthy subjects in EDTA collection tubes (vender) and centrifuged at 400 × g for 5?min at 4 °C. The cell viability of purified white blood cells (WBC) exceeded 90%.