HOMER results from RESE in OE19

Fragments of DNA capable of repressing gene expression were screened in OE19 cells, an oesophageal adenocarcinoma cell line. The screen (RESE) was carried out as described in Pang & Snyder 2020 https://www.nature.com/articles/s41588-020-0578-5.DNA regions with repressive function were determined using the computational tools MAGeCK and DESeq2. DNA motifs enriched within these regions were identified using HOMER. These DNA motifs infer the transcription factors acting at the regions of DNA.

本研究在OE19细胞系，即食道腺癌细胞系中，筛选出能够抑制基因表达的DNA片段。筛选过程（RESE）按照Pang和Snyder于2020年所述方法进行（https://www.nature.com/articles/s41588-020-0578-5）。利用计算工具MAGeCK和DESeq2确定了具有抑制功能的DNA区域。通过HOMER软件识别出这些区域中富集的DNA基序。这些DNA基序揭示了在DNA区域起作用的转录因子。