The effects of Pectobacterium brasiliense 1692 outer membrane vesicles on Phytophthora parasitica motility and metabolism

We employed a phenotypic microarray analysis to investigate the ability of <em>Phytophthora parasitica</em> INRA 310 zoospores treated with <em>Pectobacterium brasiliense </em>1692 OMVs (referred to as treated zoospores) to utilise various carbon sources provided by Biolog. These were compared to the negative control zoospores that were not treated with <em>Pbr</em>1692 OMVs (referred to as untreated zoospores). We separately added the treated and untreated zoospores into the inoculating fluid based on Table 2.1, aliquoted them into the 96 well microplates containing carbon sources at unknown concentrations, as per the manufacturer’s recommended instructions, and incubated the microplates for 48 hrs <br> The datasets also include a motility test of <em>P. parasitica</em> INRA 310 zoospores treated with <em>Pbr</em>1692 cells and <em>Pbr</em>1692 OMVs to test their effect on <em>P. parasitica</em> INRA 310 zoospores. When zoospores were tested with <em>Pbr</em>1692 cells, ddH2O was used as the negative control and and when zoospores were tested with <em>Pbr</em>1692 OMVs, PBS buffer was used as the used as a negative control. In both the experiments, <em>Pbr</em>1692 cells or OMVs were incubated with <em>P. parasitica</em> INRA 310 zoospore for 3 hours, visualising the motility of the zoospores every 30 minutes. Zoospores treated with <em>Pbr</em>1692 cells showed a reduced motility after 2 hours 30 minutes of the incubation, however, when incubated with<em> Pbr</em>1692 OMVs zoospores motility change was not observed. <br>