IL1b induction of endothelial surface IL15 by let7c3p downregulation

Expression of IL-15 on the surface of human graft endothelial cells (ECs) bound to the IL-15Rα subunit can increase the activation of CTLs, potentiating allograft rejection. Our previous work showed that surface expression of this protein complex could be induced by alloantibody-mediated complement activation through increased IL-1β synthesis, secretion, and autocrine/paracrine IL-1-mediated activation of NF-κB. In this article, we report that cultured human ECs express eight differently spliced IL-15 transcripts. Remarkably, IL-1β does not alter the expression level of any IL-15 transcript but induces surface expression independently of RNA polymerase II-mediated transcription while requiring new protein translation. Mechanistically, IL-1β causes an NF-κB-mediated reduction in the level of microRNA Let-7c-3p, thereby relieving a block of translation of IL-15 surface protein. Let7c-3p anti-miR can induce EC surface expression of IL-15/IL-15Rα in the absence of complement activation or o..., , , # IL1b induction of endothelial surface IL15 by let7c3p downregulation

Dataset DOI: [10.5061/dryad.sbcc2frgj](https://doi.org/10.5061/dryad.sbcc2frgj)

## Description of the data and file structure

qRT-PCR performed per manuscript methods description under cytokine treatments as described.

IF performed as described; captured on EVOS FL2 auto microscope. Quantified in ImageJ, reported in .xls file.

Western Blotting was performed as described in the manuscript methods.

### Files and variables

#### File: DICER_knockdown_WB_-_B-actin.png

**Description:** DICER siRNA treatment WB beta-actin loading control

#### File: DICER_knockdown_WB_-_DICER.png

**Description:**  DICER siRNA WB probe for DICER>

#### File: MFI_Data_values.xlsx

**Description:**  MFI values for quantified immunofluorescence. Please reference the associated manuscript for treatment conditions. Values tabulated on per cell measured fluorescent intensity for each relevant fluorophore.

Units: Fluorescent units

Abbrev...,