Interaction of bone marrow stroma and monocytes: bone marrow stromal cell lines cultured with monocytes

The hematopoietic microenvironment consists of non-hematopoietic derived stromal elements and hematopoietic derived monocytes and macrophages which interact and function together to control the proliferation and differentiation of early blood-forming cells. Two human stromal cell lines (HS-5 and HS-27a) representing distinct functional components of this microenvironment have been extensively characterized and shown to influence monocyte gene expression. This series of gene expression profiles is intended to extend the previous studies and identify which gene expression changes may require cell-cell contact or occur in the stromal cells as a result of monocyte influence;or in the monocytes as a result of stormal influences. Keywords: cellular response to cell contact and secreted factors Two human bone marrow stromal cell lines (HS5 and HS27a) were cultured with and without monocytes (CD14+ cells) from 2 different donors. culture condition: stroma no monocytes: HS5-1, HS5-2, HS27a-1, HS27a-2 culture condition: stroma with monocytes: HS5-MO-1, HS5-MO-2, HS27a-MO-1, HS27a-MO-2 donor: donor 3: HS5-MO-1, HS27a-MO-1 donor: donor 4: HS5-MO-2, HS27a-MO-2 cell line: HS5: HS5-1, HS5-2, HS5-MO-1, HS5-MO-2 cell line: HS27a: HS27a-1, HS27a-2, HS27a-MO-1, HS27a-MO-2