Single-cell RNA-seq Analysis of Pancreatic and Hepatic Progenitor Cells from Tg(Prox1-EGFP) Transgenic Mouse Embryos

Formation of the hepato-pancreato-biliary organ system in mammals is a paradigm for organogenesis, whereby a small progenitor population of the ventral foregut gives rise to a multitude of different adult tissues, including liver, pancreas, gallbladder, and extra-hepatic bile ducts. The multipotent ventral foregut cell population undergoes an initial fate segregation into hepatic and pancreato-biliary progenitors in response to signaling cues from surrounding mesodermal tissues. Subsequently, pancreato-biliary progenitors give rise to ventral pancreatic and gallbladder progenitors. In this study, we used single-cell RNA sequencing to molecularly characterize progenitor populations in the ventral foregut that contribute to the formation of hepatic, pancreatic, and biliary organ rudiments throughout organogenesis. 452 single cells isolated from foregut, pancreatic and hepatic progenitor cells of Tg(Prox1-EGFP) transgenic mouse embryos at embryonic day (E) 8.5, 10.5, and E14.5 were processed for single-cell RNA sequencing using SmartSeq2 method. Only samples that passed the QC check are included in this submission.哺乳动物肝胆胰（hepato-pancreato-biliary）器官系统的形成是器官发生研究的经典范式：腹侧前肠（ventral foregut）的一小群祖细胞可分化为肝脏、胰腺、胆囊及肝外胆管等多种成体组织。这群具有多能性的腹侧前肠细胞群会响应周围中胚层组织的信号提示，初步发生命运分化，分为肝祖细胞与肝胆胰祖细胞。随后，肝胆胰祖细胞进一步分化为腹侧胰腺祖细胞与胆囊祖细胞。本研究采用单细胞RNA测序（single-cell RNA sequencing）技术，对参与整个器官发生过程中肝、胰及胆道器官原基形成的腹侧前肠祖细胞群进行分子特征解析。本研究共从Tg(Prox1-EGFP)转基因小鼠胚胎（胚胎发育第8.5天、10.5天及14.5天）的前肠、胰腺及肝祖细胞中分离得到452个单细胞，并采用SmartSeq2方法完成单细胞RNA测序。本次提交仅纳入通过质量控制（QC）检测的样本。