Chromatin analysis of multiple myeloma cells following CBP/p300 inhibition and degradation (ChIP-Seq)

We performed H3K27ac ChIP-seq on MM.1S cells treated with A-485, GNE-781, and the combination of both, or with dCBP-1, and CBP & p300 ChIP on MM.1S cells. Cells were treated for six hours with each inhibitor (or combination) at a concentration of 100 nM. Cells were then crosslinked (for ChIP-seq), lysed with appropriate lysis buffers, and ChIP-seq procedure was carried out.