RNA-Seq of Bovine Precursor Adipocytes

The HGFAC gene was subjected to deletion and acquisition assays, respectively, and RNA-Seq was performed in bovine precursor adipocytes transfected with si-NC, si-HGFAC, pcDNA3.1-NC, and pcDNA3.1-HGFAC, respectively, and by comparing the groups of si-NC, pcDNA3.1-NC, si-HGFAC, and pcDNA3.1-HGFAC, respectively, the si-HGFAC, pcDNA3.1-HGFAC and pcDNA3.1-HGFAC groups were identified. HGFAC, 46 and 130 differentially expressed genes were identified, respectively. Among them, in the si-HGFAC group, 33 genes were up-regulated and 13 genes were down-regulated; in the pcDNA3.1-HGFAC group, 27 genes were up-regulated and 103 genes were down-regulated, and the screened differentially expressed genes were subsequently analyzed by GO and KEGG. Interference assay: Bovine precursor adipocytes were subjected to transfection assay, divided into NC group and si group, with three replicates in each group. 48h after transfection, the cells were collected by Trizol, frozen and sequenced. Overexpression assay: bovine precursor adipocytes were transfected with pcDNA3.1, divided into pcDNA3.1-NC group and pcDNA3.1-HGFAC, three replicates in each group, 48h after transfection, cells were collected by Trizol, frozen and sequenced.