Acute hypoxia changes the utilization mode of glucose / lipid in the liver of the largemouth bass (Micropterus salmoides)

In the present study, we used NGST to characterize mRNA-seq of control-, moderate hypoxia-treated and severe hypoxia-treated Micropterus salmoides livers to elucidate the molecular mechanisms of hypoxia adaptation. This is the first report on integrated analysis of the tissue specific and temporal changes in gene expression in largemouth bass (Micropterus salmoides) exposed to hypoxia could reveal mechanisms of hypoxia adaptation. We provide a good case study with which to analyse mRNA expression and profile non-model fish species using NGST. We used RNA sequencing to detect mRNA transcriptomes of liver from largemouth bass with moderate hypoxia group (MHG) and severe hypoxia group (SHG) compared them with control group (CG) under 0h, 4h, 8h, 12h, and 24h (3 vs. 3).