Different real-time LCR protocols tested.
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Different thermocycling conditions, DNA ligases, length of oligonucleotides, gap modifications were examined. All trials were performed with the Mx3005P QPCR platform. Gap-A and gap-T LCR protocols used Platinum® Taq DNA polymerase.aOligonucleotides used LCPR1, LCPR2, LCPR3s, LCPR4s.bOligonucleotides used LCPR1, LCPR2, LCPR3L, LCPR4L.cOligonucleotides used LCPR1, LCPR2G, LCPR3s, LCPR4s.dOligonucleotides used LCPR1, LCPR2, LCPR3G, LCPR4s.eOptimal real-time LCR protocol.fThe LOD and LOQ values are in % frequencies
创建时间：
2015-12-02
