miRNA profiling of rice developing leaves in response to elevated CO2

Elevated CO2 (eCO2) has an influence on developing leaf growth of rice (Oryza sativa cv. Nipponbare), specifically lower growth stage than P4 (plastochron number), resulting in decrease in leaf size compared with that in ambient CO2 (aCO2). Since several micro RNAs are associated with the regulation of plant leaf development, in order to clarify which micro RNAs are involved in the decrease of leaf blade size at eCO2, we carried out high-throughput small RNA sequencing analysis and compared the amount of identified miRNAs in developing rice leaf blade grown between aCO2 and eCO2 condition. Total RNAs were isolated from rice developing leaf at 7 h and 13.5 h after elevated CO2 treatment on daytime. Two independent experiments were performed at each time (7 and 13.5 h) using different samples.