Correlates of protection in macaques vaccinated with rBmHAT.

The three vaccinated macaques that did not show any evidence of infection had high titer of anti- rBmHAT IgG antibodies and their PBMC secreted significantly high levels of IFN-γ but low levels of IL-10 as determined by an ELISPOT assay. Ratio of IFN-γ: IL-10 secreting cells was calculated for each macaque. These analyses also confirmed that there is a clear difference between the infected macaques and the three vaccinated macaques that did not show any infection. These findings coupled with the ADCC data clearly suggest that the three vaccinated macaques that did not show any signs of infection were protected.#PBMC collected before challenge and stimulated with rBmHAT.Correlates of protection in macaques vaccinated with rBmHAT.