H. pylori 26695 WT / AGS

Tilt Series Date: 2016-11-02 Data Taken By: Yi-Wei Chang Species / Specimen: Helicobacter pylori Strain: 26695 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Strain and sample prepared by Carrie Shaffer Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ml gold+ 25 ml 10% BSA x4, resuspend in 30 ul). Blot for 5 sec (1 sec longer than usual to remove more solution). Ethane/propane mix. ycw2016-11-02-8_slicer17150.jpg: cag T4SS tubes? ycw2016-11-02-8_slicer17151.jpg: tube periodicity (pink) and cross sections (green) ycw2016-11-02-8_slicer17152.jpg: tube cross sections ycw2016-11-02-8_slicer17153.jpg: long tube on top of H. pylori cell ycw2016-11-02-8_slicer17871.jpg: cag T4SS wi fi particle?