The quantitative analysis of transcriptomes derived from malignant rhabdoid tumor cells treated with EZH1/2 dual inhibitor or EZH2 selective inhibitor

We found that both EZH1 and EZH2 contributes to cell growth and H3K27 methylation, and EZH1/2 dual inhibitors induce growth inhibition in malignant rhabdoid tumor (MRT). To investigate the molecular mechanism underlying the growth inhibition of EZH inhibitors in MRT cells, we performed RNA-sequencing analysis and compared differentially expressed genes between A204.1 cells treated with EZH1/2 dual inhibitor DS-3201b or EZH2 selective inhibitor EPZ-6438. Overall design: The comparisons of transcriptomes among EZH inhibitors (DS-3201b or EPZ-6438)-treated and non-treated (Control) A204.1 or TTC549 cells. A204.1 or TTC549 cells were treated with DS-3201b (100 nM) or EPZ-6438 (100 nM) for 14 days, followed by RNA-sequencing (RNA-seq) analysis.