RNA sequencing of rice with mutation of OsSPL4 by CRISPR/Cas9. Oryza sativa

Grain weight and grain number, the two important yield traits, are mainly determined by grain size and panicle architecture in rice. Herein, we report the identification and functional analysis of OsSPL4 in panicle and grain development of rice. Using CRISPR/Cas9 system, the mutation (spl4-d3) of OsSPL4 was obtained, and the flag leaves and young panicles were used for RNA sequencing (RNA-seq).