Effect of binary Ti vectors pSiM24 and pSiM24-GUS/GFP on transformation frequencies in <i>A. thaliana</i>.
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A. tumefaciens harboring binary vectors were cultured and floral dipping of A. thaliana plants was subsequently performed as described in materials and methods. T1 seedlings were selected on solid MS medium containing kanamycin. The data was obtained from at least four independent lines developed for each construct. Statistical analysis of the data was performed adopting one way ANOVA analysis (using GraphPad Prism version 5.01) and presented as the means ± S.D. A P value of less than 0.05 was considered significant indicated by different superscript letters.
创建时间:
2014-06-04



