基于转录组学分析广叶绣球菌多糖改善小鼠骨骼肌糖代谢紊乱机制

The study was designed to investigate the improvement effect and molecular mechanism of Sparassis latifolia polysaccharide (SLPs) on glucose metabolism in skeletal muscle of mice with glucose metabolism disorder induced by high-fat diet combined with streptozotocin (STZ). The results showed that SLPs intervention could significantly reduce the impaired glucose tolerance and insulin resistance and increase skeletal muscle glycogen content and improve the morphological damage of skeletal muscle caused by glucose metabolism disorder. Transcriptome analysis identified a total of 965 differentially expressed genes (DEGs). GO annotation and enrichment analysis showed that these genes were mainly involved in tissue development, animal organ morphogenesis, small molecule metabolism, myofibril, glycogen binding, and organic acid binding. The enrichment analysis of KEGG further revealed that DEGs were mainly enriched in PI3k/Akt signal pathway, AGE-RAGE signal pathway related to diabetic complications, insulin resistance, AMPK signal pathway and other signal pathways. In addition, SLPs could regulate the expression of genes and proteins related to AMPK/SIRT1/PGC-1α signal pathway and the activities of key enzymes in skeletal muscle of mice to improve glucose metabolism disorder in skeletal muscle, protect skeletal muscle damage, and maintain the metabolic homeostasis of the body. This study provides a theoretical basis for the development of SLPs as functional food ingredients.