Chromosome editing with trisomic chromosome in chicken DT40 cells

A trisomy is a type of aneuploidy characterised by an additional chromosome. The additional chromosome theoretically accepts any kind of changes since it is not necessary for cellular proliferation. This advantage led us to apply two chromosome manipulation methods to autosomal trisomy in chicken DT40 cells. We first corrected chromosome 2 trisomy to disomy by employing counter-selection markers. We next tested large-scale truncations of chromosome 2 to make a mini-chromosome by introducing telomere repeat sequences to delete most of p-arm or q-arm of chromosome 2. Successful establishment of cell lines were confirmed by whole-genome sequence analysis.