Novel Targets for Diabetic Foot Ulcer Healing: A Transcriptomic Analysis by Negative Pressure Wound Therapy

The results of the present study provide new insights into the mechanism through which NPWT promotes DFU wound healing Participants underwent wound debridement to remove blackened and necrotic soft and bone tissues, followed by NPWT. For NPWT, a vacuum assisted closure (VAC) negative pressure-assisted healing therapy system (KCI, USA) was used according to published protocols , at a dose of -125 mmHg for 1 week. Full-thickness skin tissue was taken within 0.5 cm of the wound edge before and after 1 week of NPWT, only after removing the negative pressure device. The collected granulation tissue was stored at -80ºC for examination.RNA-seq was performed on the granulation tissue of three patients with DFU before and after NPWT (before/after-patient 1, before/after-patient 2, before/after-patient 3)