John Murray (University of Pennsylvania), Ke Hu (Indiana University, Bloomington) (2011) CIL:10534, Toxoplasma gondii RH. CIL. Dataset

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged Tgbeta3-tubulin. Images acquired using API DeltaVision on an Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: GFP, ex 450-490 em 500-540. Captured with CoolSnapHQ/ICX285. Deconvolved stack corresponds to raw data stack: CIL# 10533.

Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

一层人类皮肤成纤维细胞被感染了表达EGFP标记的Tgbeta3-微管蛋白的弓形虫。利用Olympus IX70倒置显微镜上的DeltaVision API获取图像。实验目的：使用Olympus 60X水浸式物镜，数值孔径为1.2。滤光片：GFP滤光片，激发波长450-490nm，发射波长500-540nm。图像通过CoolSnapHQ/ICX285捕获。去卷积后的图像堆栈对应于原始数据堆栈：CIL# 10533。此图像为未发表图像，与PMID: 16518471发表的图像相似。有关其他实验细节，请参阅相关出版物。