Detailed analysis of the murine-aged brain transcriptome [ONT]

In this work, we collected and analyzed two cohorts of young-adult and aged-adult mice brain mRNAs and determined their levels using second- (illumina) and third-generation (Oxford Nanopore) sequencing technologies. We report a transcriptome-wide study of differential transcript usage during brain aging. In addition, we provide the community with a large resource of whole brain transcriptomes and comprehensive analyses that identify widespread diversity of mRNAs during aging. Overall design: Brain mRNA profiles of 4 young-adult samples (6-month, brain hemisphere, from 4 males), 4 aged (24-month, brain hemisphere, from 4 males)